Robert J. Stanley
A.B. ('78), University of California - Berkeley
Biological / Photonics / Physical
A principal focus of our group is to understand the mechanism of DNA repair by the light-driven enzyme, DNA photolyase. DNA is often damaged by UV radiation that is not otherwise absorbed by the ozone layer. Photolyase is a FAD-containing (flavo)protein that uses light to drive an ultrafast electron transfer reaction between the protein and the bound DNA lesion. The transferred electron repairs the damaged DNA by an unknown mechanism. We are using ultrafast laser and biochemical techniques to unravel this mechanism. We also explore the details of substrate binding using state of the art fluorescence reporter and two photon excitation techniques.
Pauszek, Raymond F. and Stanley, Robert J., "A "How-To Guide to the Stark Spectroscopy of Flavins and Flavoproteins", Edited by: Weber, S; Schleicher, E, FLAVINS AND FLAVOPROTEINS: METHODS AND PROTOCOLS Book Series: Methods in Molecular Biology, (2014), 1146, 443-466.
Pauszek, Raymond F., III; Kodali, Goutham; Stanley, Robert J., "Excited State Electronic Structures of 5,10-Methenyltetrahydrofolate 5,10-Methylenetetrahydrofolate Determined by Stark Spectroscopy", J PHYS CHEM A, (2014), 118, 8320-8328.
Pauszek, R. F., III; Kodali, G.; Caldwell, S. T.; Stanley, R. J. "Excited State Charge Redistribution and Dynamics in the Donor-pi-Acceptor Flavin Derivative ABFL" Journal of Physical Chemistry B (2013), 117 (49), 15684-15694.
Pauszek, R. F., III; Kodali, G.; Siddiqui, M., Salim; Stanley, R. J. "Investigation of Excited State Charge Redistribute of the Reduced Anionic Flavin in DNA Photolyase and Simple Solvents by Stark Spectroscopy" Biophysical Journal (2013), 104 (2), 488A-488A.
Munshi, S.; Stanley, R. J. "Extremophile Enzymatic DNA Repair" Biophysical Journal (2013), 104 (2), 490A-490A.
Narayanan, M.; Kodali, G.; Singh, V. R.; Stanley, R. J. "Oxidation and reduction potentials of 8- vinyladenosine measured by cyclic voltammetry: quenching of a fluorescent adenine analog" Journal of Photochemistry and Photobiology A-Chemistry (2012), 249, 9-14.
Yu, X.; Eymur, S.; Singh, V.; Yang, B. Q.; Tonga, M.; Bheemaraju, A.; Cooke, G.; Subramani, C.; Venkataraman, D.; Stanley, R. J.; Rotello, V. M. "Flavin as a photo-active acceptor for efficient energy and charge transfer in a model donor-acceptor system." Physical Chemistry Chemical Physics (2012), 14 (19), 6749-6754.
Narayanan, M.; Kodali, G.; Xing, Y.; Stanley, R. J. "Photoinduced
electron transfer occurs between 2-aminopurine and the DNA nucleic acid
monophosphates: results from cyclic voltammetry and fluorescence
quenching." Journal of Physical Chemistry B (2010), 114 (32), 10573-10580.
Narayanan, M.; Kodali, G.; Xing, Y.; Hawkins, M. E.; Stanley, R.
J. "Differential Fluorescence Quenching of Fluorescent Nucleic Acid Base
Analogues by Native Nucleic Acid Monophosphates." The Journal of
Physical Chemistry B (2010), 114 (17), 5953-5963.