Robert J. Stanley
A.B. ('78), University of California - Berkeley
Biological / Photonics / Physical
A principal focus of our group is to understand the mechanism of DNA repair by the light-driven enzyme, DNA photolyase. DNA is often damaged by UV radiation that is not otherwise absorbed by the ozone layer. Photolyase is a FAD-containing (flavo)protein that uses light to drive an ultrafast electron transfer reaction between the protein and the bound DNA lesion. The transferred electron repairs the damaged DNA by an unknown mechanism. We are using ultrafast laser and biochemical techniques to unravel this mechanism. We also explore the details of substrate binding using state of the art fluorescence reporter and two photon excitation techniques.
Yu, X.; Eymur, S.; Singh, V.; Yang, B. Q.; Tonga, M.; Bheemaraju, A.; Cooke, G.; Subramani, C.; Venkataraman, D.; Stanley, R. J.; Rotello, V. M. "Flavin as a photo-active acceptor for efficient energy and charge transfer in a model donor-acceptor system." Physical Chemistry Chemical Physics (2012), 14 (19), 6749-6754.
Narayanan, M.; Kodali, G.; Xing, Y.; Stanley, R. J. "Photoinduced
electron transfer occurs between 2-aminopurine and the DNA nucleic acid
monophosphates: results from cyclic voltammetry and fluorescence
quenching." Journal of Physical Chemistry B (2010), 114 (32), 10573-10580.
Narayanan, M.; Kodali, G.; Xing, Y.; Hawkins, M. E.; Stanley, R.
J. "Differential Fluorescence Quenching of Fluorescent Nucleic Acid Base
Analogues by Native Nucleic Acid Monophosphates." The Journal of
Physical Chemistry B (2010), 114 (17), 5953-5963.
Kodali, G.; Siddiqui, S. U.; Stanley, R. J. "Charge Redistribution
in Oxidized and Semiquinone E. coli DNA Photolyase upon
Photoexcitation: Stark Spectroscopy Reveals a Rationale for the Position
of Trp382." J. Am. Chem. Soc. (2009), 131 (13), 4795-4807.
Yang, K.; Matsika, S.; Stanley, R. J. "6MAP, a fluorescent adenine
analogue, is a probe of base flipping by DNA photolyase." Journal of
Physical Chemistry, B (2007), 111 (35), 10615-10625.
Stanley, R. J.; Hou, Z.; Yang, A.; Hawkins, M. E. "The Two-Photon Excitation Cross Section of 6MAP, a Fluorescent Adenine Analogue." Journal of Physical Chemistry B (2005), 109 (8), 3690-3695.
Christine, K. S.; MacFarlane IV, A. W.; Yang, K.; Stanley, R. J. "Cyclobutylpyrimidine Dimer Base Flipping by DNA Photolyase." Journal of Biological Chemistry (2002), 277 (41), 38339-38344.