Our lab studies the mechanisms of enzymatic reactions necessary for maintaining the information stored in DNA and RNA. This work aids in drug design, the development of new biotechnological tools, and in the innovation of diagnostic technologies. Our lab is highly interdisciplinary. Students learn approaches and techniques in enzymatic catalysis, macromolecular interactions, protein expression/purification, reconstitution of biochemical reactions, biophysics, mechanistic biochemistry, structural biochemistry, and in vivo biological assays.
Yoori Kim, Christopher M. Furman, Carol M. Manhart, Eric Alani, and Ilya Finkelstein. Intrinsically disordered regions regulate both catalytic and non-catalytic activities of the MutL𝜶 mismatch repair complex, Nucleic Acids Research, 2019, 47 (4): 1823-183.
Carol M. Manhart, Xiaodan Ni, Martin A. White, Joaquin Ortega, Jennifer Surtees, and Eric Alani. The mismatch repair and meiotic recombination endonuclease Mlh1-Mlh3 is activated by polymer formation and can cleave DNA substrates in trans, PLoS Biology, 2017, 15 (4): e2001164.
Carol M. Manhart and Eric Alani. Roles for mismatch repair family proteins in promoting meiotic crossing over, DNA Repair (Special Issue), invited review article, 2016, 38: 84-93.
Carol M. Manhart and Charles S. McHenry. Identification of subunit binding positions on a model fork and displacements that occur during sequential assembly of the Escherichia coli primosome, Journal of Biological Chemistry, 2015, 290 (17): 10828-10839.
Maria V. Rogacheva*, Carol M. Manhart*, Cheng Chen, Alba Guarné, Jennifer Surtees, and Eric Alani. Mlh1-Mlh3, a meiotic crossover and DNA mismatch repair factor, is a Msh2-Msh3-stimulated endonuclease, Journal of Biological Chemistry, 2014, 289 (9): 5664-5673. [*contributed equally to this work]
Paul R. Dohrmann, Carol M. Manhart, Christopher D. Downey, and Charles S. McHenry. The rate of polymerase release upon filling the gap between Okazaki fragments is inadequate to support cycling during lagging strand synthesis, Journal of Molecular Biology, 2011, 414 (1): 15-27.