Research in our lab lies at the interface of chemistry and biology, with an eye towards understanding and treating human diseases. We use interdisciplinary approaches such as organic synthesis, biochemistry, chemical proteomics, protein engineering, cell biology, and molecular imaging to investigate the biological mechanisms underlying post-translational modifications that are key to cancer, neuron degeneration, and immune disorders. In particular, we are interested in synthetic chemistry methodology for novel bioorthogonal reactions, probe design and application for dissection of post-translational modifications on proteins. We are also actively inventing novel diagnostics and therapeutics, using our expertise in chemical synthesis, protein engineering, drug delivery, library screening, cell assay, and animal models. Students have the opportunity to work in various areas spanning from organic synthesis to molecular biology/cell biology. The training is expected to help students achieve well-rounded skill sets and a strong publication record in either chemistry, biology, or chemical biology.
Unprotected Peptide Macrocyclization and Stapling via a Fluorine-Thiol Displacement Reaction
Islam MS, Junod SL, Zhang S, Buuh ZY, Guan Y, Zhao M, Kaneria KH, Kafley P, Cohen C, Maloney R, Lyu Z, Voelz V, Yang W, Wang RE.
Nature Commun. 2022, 13(1): 350
Steric-Free Bioorthogonal Labeling of Acetylation Substrates Based on a Fluorine-Thiol Displacement Reaction.
Lyu Z, Zhao Y, Buuh ZY, Gorman N, Goldman AR, Islam MS, Tang HY, Wang RE.
J Am Chem Soc. 2021 Jan 12;. doi: 10.1021/jacs.0c05605
Site-specific antibody fragment conjugates for targeted imaging.
Maloney R, Buuh ZY, Zhao Y, Wang RE. Methods Enzymol. 2020;638:295-320.
A tumor-targeted immune checkpoint blocker
Zhang Y, Fang C, Wang RE, Wang Y, Guo H, Guo C, Zhao L, Li S, Li X, Schultz PG, Cao YJ, Wang F. Proc Natl Acad Sci U S A. 2019 Aug 6;116(32):15889-15894.
Site-Specific Immuno-PET Tracer to Image PD-L1.
Wissler HL, Ehlerding EB, Lyu Z, Zhao Y, Zhang S, Eshraghi A, Buuh ZY, McGuth JC, Guan Y, Engle JW, Bartlett SJ, Voelz VA, Cai W, Wang RE. Mol Pharm. 2019 May 6;16(5):2028-2036.
A Switchable Site-Specific Antibody Conjugate.
Lyu Z, Kang L, Buuh ZY, Jiang D, McGuth JC, Du J, Wissler HL, Cai W, Wang RE.
ACS Chem Biol. 2018 Apr 20;13(4):958-964.
Interrogating the Roles of Post-Translational Modifications of Non-Histone Proteins.
Buuh ZY, Lyu Z, Wang RE.
J Med Chem. 2018 Apr 26;61(8):3239-3252